WNT4 Antibody (Center)

APR05783G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05783G		100 μl / ￥4950

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WNT4 Antibody (Center)

品牌	Leading Biology	貨號(hào)	APR05783G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality WNT4 Antibody (Center).
分子量	39052 Da
細(xì)胞定位	Antigen Cellular Localization: Secreted, extracellular space, extracellular matrix
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse, Rat
免疫原	211-239 aa
靶點(diǎn)	This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.
亞型	Rabbit Ig
基因ID	54361
UniProt ID	P56705
功能	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
總結(jié)	The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
圖像	All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: mouse bladder lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lane 3: mouse bladder lysate Lane 4: rat lung lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of WNT4 Antibody (Center) (Cat. APR05783G) in mouse bladder tissue lysates (35ug/lane).WNT4 (arrow) was detected using the purified Pab.
說明書	Product Datasheet Protocol
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