品牌 |
Leading Biology |
貨號 |
APR04934G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDC80 Antibody (C-Term).
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分子量 |
73913 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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靶點 |
This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 594-628 amino acids from human NDC80.
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亞型 |
Rabbit Ig
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通用名 |
HEC, HEC1, KNTC2
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基因ID |
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UniProt ID |
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功能 |
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
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總結(jié) |
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:2000
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圖像 |
All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: 293T/17 whole cell lysate
Lane 6: Jurkat whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 74 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數(shù)量 |
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