FOXO4 Antibody (Center)

APR04859G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04859G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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FOXO4 Antibody (Center)

品牌	Leading Biology	貨號	APR04859G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FOXO4 Antibody (Center).
分子量	53684 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm
宿主	Rabbit
種屬反應性	Human
靶點	This FOXO4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 206-240 amino acids from the Central region of human FOXO4.
亞型	Rabbit Ig
通用名	AFX, AFX1, MLLT7
基因ID	4303
UniProt ID	P98177
功能	Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
總結(jié)	Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	IF, WB, E
稀釋方法	IF~~1:25 WB~~1:1000-1:2000
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with APR04859G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). All lanes : Anti-FOXO4 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: K562 whole cell lysates Lane 3: Ramos whole cell lysates Lane 4: NCI-H1299 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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