MAD2L2 Antibody (C-term)

APR04652G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04652G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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MAD2L2 Antibody (C-term)

品牌	Leading Biology	貨號	APR04652G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MAD2L2 Antibody (C-term).
分子量	24334 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm
宿主	Rabbit
種屬反應(yīng)性	Mouse
靶點	This MAD2L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-231 amino acids from the C-terminal region of human MAD2L2.
亞型	Rabbit Ig
通用名	MAD2B, REV7
基因ID	10459
UniProt ID	Q9UI95
功能	Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
總結(jié)	Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	IF~~1:25 IHC-P~~1:25 WB~~1:1000
圖像	Fluorescent image of Hela cells stained with MAD2L2 Antibody (C-term)( APR04652G). APR04652G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Immunohistochemical analysis of paraffin-embedded H. kidney section using MAD2L2 Antibody (C-term)( APR04652G). APR04652G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Western blot analysis of lysates from Hela, K562 cell line (from left to right), using MAD2L2 Antibody (C-term)(APR04652G). APR04652G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
說明書	Product Datasheet Protocol
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