Connexin 43 Antibody (N-term)

APR04054G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04054G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Connexin 43 Antibody (N-term)

品牌	Leading Biology	貨號	APR04054G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Connexin 43 Antibody (N-term).
分子量	43008 Da
細胞定位	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction
宿主	Rabbit
種屬反應性	Human, Mouse, Rat
免疫原	107-138 aa
靶點	This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
亞型	Rabbit Ig
通用名	GJAL
基因ID	2697
UniProt ID	P17302
功能	Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity).
總結	Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
圖像	Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121)(APR04054G). APR04054G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. (LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(Cat.APR04054G) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin (Cat.APR04054G) in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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