CD9 Antibody (Center)

APR04023G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04023G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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CD9 Antibody (Center)

品牌	Leading Biology	貨號	APR04023G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD9 Antibody (Center).
分子量	25416 Da
細胞定位	Antigen Cellular Localization: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
宿主	Rabbit
種屬反應性	Human
免疫原	115-145 aa
靶點	This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the Central region of human CD9.
亞型	Rabbit Ig
通用名	MIC3, TSPAN29
基因ID	928
UniProt ID	P21926
功能	Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
總結	CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	IF, WB, FC, E
稀釋方法	IF~~1:25 WB~~1:1000 FC~~1:10~50
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with APR04023G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line. The nuclear counter stain is DAPI (blue). Anti-CD9 Antibody (Center)at 1:2000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center)(APR04023G). APR04023G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
說明書	Product Datasheet Protocol
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