品牌 |
Leading Biology |
貨號 |
APR06133G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CDC37 Antibody (Center).
|
分子量 |
44468 Da
|
細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm.
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human, Rat
|
免疫原 |
116-144 aa
|
靶點 |
This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37.
|
亞型 |
Rabbit Ig
|
通用名 |
CDC37A
|
基因ID |
|
UniProt ID |
|
功能 |
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
|
總結(jié) |
CDC37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, IHC-P, FC, E
|
稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
|
圖像 |
All lanes : Anti-CDC37 Antibody (Center) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: C6 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: LNCaP whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of CDC37 Antibody (Center) (Cat. APR06133G) in MCF-7, A2058 cell line lysates (35ug/lane). CDC37 (arrow) was detected using the purified Pab.
CDC37 Antibody (Center) (Cat. APR06133G) IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDC37 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
|
說明書 |
|
數(shù)量 |
|