品牌 |
Leading Biology | 貨號(hào) |
APR06132G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CLIP1 Antibody (N-term).
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分子量 |
162246 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
228-254 aa
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靶點(diǎn) |
This CLIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the N-terminal region of human CLIP1.
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亞型 |
Rabbit Ig
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通用名 |
CYLN1, RSN
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基因ID |
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UniProt ID |
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功能 |
Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
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總結(jié) |
CLIP1 may be a intermediate filament associated protein that links endocytic vesicles to microtubules.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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圖像 |
Anti-CLIP1 Antibody (N-term) at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 162 kDa Blocking/Dilution buffer: 5% NFDM/TBST. CLIP1 Antibody (N-term) (Cat. APR06132G) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. CLIP1 Antibody (N-term) (Cat. APR06132G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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