品牌 |
Leading Biology |
貨號 |
APR06128G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GPA33 Antibody (C-term).
|
分子量 |
35632 Da
|
細(xì)胞定位 |
Antigen Cellular Localization:
Membrane; Single-pass type I membrane protein
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human
|
免疫原 |
264-293 aa
|
靶點(diǎn) |
This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33.
|
亞型 |
Rabbit Ig
|
基因ID |
|
UniProt ID |
|
功能 |
May play a role in cell-cell recognition and signaling.
|
總結(jié) |
GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, IHC-P, FC, E
|
稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
|
圖像 |
All lanes : Anti-GPA33 Antibody (C-term) at 1:1000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: HT-29 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of GPA33 Antibody (C-term) (Cat. APR06128G) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.
GPA33 Antibody (C-term) (Cat. APR06128G) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
|
說明書 |
|
數(shù)量 |
|