品牌 |
Leading Biology | 貨號 |
APR06048G |
產品分類 |
Polyclonal Antibodies | 研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CYP3A43 Antibody (Center).
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分子量 |
57670 Da
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細胞定位 |
Antigen Cellular Localization:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
242-270 aa
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靶點 |
This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Exhibits low testosterone 6-beta-hydroxylase activity.
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總結 |
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IF, FC, E
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稀釋方法 |
WB~~1:2000
FC~~1:10~50
IF~~1:10~50
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圖像 |
Anti-CYP3A43 Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. CYP3A43 Antibody (Center) (Cat. APR06048G) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Confocal immunofluorescent analysis of CYP3A43 Antibody (Center)( APR06048G) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說明書 |
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數(shù)量 |
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