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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > TRIB1 Antibody (C-term)

TRIB1 Antibody (C-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
APR06003G 100 μl / ￥4950

TRIB1 Antibody (C-term)

品牌

 Leading Biology

貨號(hào)

 APR06003G

產(chǎn)品分類

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TRIB1 Antibody (C-term).

分子量
41009 Da

宿主
Rabbit

種屬反應(yīng)性
Human

免疫原
341-370 aa

靶點(diǎn)
This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1.

亞型
Rabbit Ig

基因ID

UniProt ID

功能
Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.

總結(jié)
TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities.Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, E

稀釋方法
WB~~1:1000

圖像

Anti-TRIB1 Antibody C-term at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TRIB1 Antibody C-term at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-TRIB1 Pab (APR06003G) in T47D cell line lysate (35ug/lane). TRIB1(arrow) was detected using the purified Pab.

說(shuō)明書(shū)

數(shù)量

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