品牌 |
Leading Biology | 貨號(hào) |
APR05893G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SMYD3 Antibody (Center).
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分子量 |
49097 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
209-236 aa
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靶點(diǎn) |
This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.
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亞型 |
Rabbit Ig
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通用名 |
ZMYND1, ZNFN3A1
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基因ID |
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UniProt ID |
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功能 |
Histone methyltransferase. Specifically methylates 'Lys- 4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
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總結(jié) |
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
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圖像 |
Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center)(APR05893G). APR05893G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. Western blot analysis of SMYD3 antibody (Center) (Cat.APR05893G) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab. SMYD3 Antibody (Center) (Cat. APR05893G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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