HSP27 (HSPB1) Antibody (S83)

APR05850G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05850G		100 μl / ￥4950

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HSP27 (HSPB1) Antibody (S83)

品牌	Leading Biology	貨號(hào)	APR05850G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HSP27 (HSPB1) Antibody (S83).
分子量	22783 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	61-90 aa
靶點(diǎn)	This HSP27(HSPB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from human HSP27(HSPB1).
亞型	Rabbit Ig
通用名	HSP27, HSP28
基因ID	3315
UniProt ID	P04792
功能	Involved in stress resistance and actin organization.
總結(jié)	In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	Western blot analysis of HSPB1 Antibody (S83) (Cat.APR05850G) in Hela cell line lysates (35ug/lane). HSPB1 (arrow) was detected using the purified Pab. Western blot analysis of HSPB1(arrow) using rabbit polyclonal HSPB1 Antibody (S83) (Cat.APR05850G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
說明書	Product Datasheet Protocol
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