PBK Antibody (C-term C300)

APR05849G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05849G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PBK Antibody (C-term C300)

品牌	Leading Biology	貨號(hào)	APR05849G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PBK Antibody (C-term C300).
分子量	36085 Da
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	286-317 aa
靶點(diǎn)	This PBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-317 amino acids from the C-terminal region of human PBK.
亞型	Rabbit Ig
通用名	TOPK
基因ID	55872
UniProt ID	Q96KB5
功能	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
總結(jié)	PBK is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	Anti-PBK/TOPK Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300) (Cat.APR05849G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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