CAMKK2 Antibody (C-term)

APR05845G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05845G		100 μl / ￥4950

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CAMKK2 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR05845G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAMKK2 Antibody (C-term).
分子量	64746 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells (By similarity). Found in the cytoplasm and neurites after forskolin induction.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	483-512 aa
靶點(diǎn)	This CAMKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of human CAMKK2.
亞型	Rabbit Ig
通用名	CAMKKB, KIAA0787
基因ID	10645
UniProt ID	Q96RR4
功能	Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Efficiently phosphorylates 5'-AMP- activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
總結(jié)	CAMKK2 belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 appears to be involved in hippocampal activation of CREB1.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50
圖像	Western blot analysis of CAMKK2 (arrow) using rabbit polyclonal CAMKK2 Antibody (C-term)(Cat.APR05845G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMKK2 antibody (C-term)(Cat.AP711b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說(shuō)明書	Product Datasheet Protocol
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