CALD1 Antibody (Center)

APR05773G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05773G		100 μl / ￥4950

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CALD1 Antibody (Center)

品牌	Leading Biology	貨號(hào)	APR05773G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CALD1 Antibody (Center).
分子量	93231 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle)
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	428-457 aa
靶點(diǎn)	This CALD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the Central region of human CALD1.
亞型	Rabbit Ig
通用名	CAD, CDM
基因ID	800
UniProt ID	Q05682
功能	Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
總結(jié)	CALD1 is a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
圖像	Western blot analysis of CALD1 antibody (Center) (Cat. APR05773G) in A2058 cell line lysates (35ug/lane). CALD1 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human lung carcinoma with CALD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of NCI-H292 cells using CALD1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說(shuō)明書(shū)	Product Datasheet Protocol
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