EEF1A1 Antibody (C-term)

APR05772G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05772G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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EEF1A1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR05772G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EEF1A1 Antibody (C-term).
分子量	50141 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	361-389 aa
靶點(diǎn)	This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
亞型	Rabbit Ig
通用名	EEF1A, EF1A, LENG7
基因ID	1915
UniProt ID	P68104
功能	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
總結(jié)	EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IF, IHC-P, E
稀釋方法	WB~~1:1000 FC~~1:10~50 IF~~1:10~50 IHC-P~~1:10~50
圖像	Western blot analysis of EEF1A1 Antibody (C-term) (Cat. APR05772G) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab. Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term)( APR05772G) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
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