品牌 |
Leading Biology | 貨號 |
APR05512G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality BTG1 Antibody (Center).
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分子量 |
19209 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
92-118 aa
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靶點(diǎn) |
This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Anti-proliferative protein.
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總結(jié) |
BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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圖像 |
All lanes : Anti-BTG1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST. (LEFT)Western blot analysis of BTG1 Antibody (Center) (Cat. APR05512G) in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody (Center) (Cat. APR05512G) in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab. BTG1 Antibody (Center) (Cat. APR05512G) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTG1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
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說明書 |
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數(shù)量 |
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