品牌 |
Leading Biology | 貨號(hào) |
APR05478G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Fibrinogen beta chain Antibody.
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分子量 |
51; [uniprot,origene,epi]56; KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Secreted.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat, Chicken, Dog, Guinea Pig, Rabbit
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免疫原 |
401-491/491 aa
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基因ID |
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UniProt ID |
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功能 |
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
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總結(jié) |
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC, IF
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稀釋方法 |
IF~~1:100~1:200
WB~~ 1:500
IHC~~1:100-1:500
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圖像 |
Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Fibrinogen beta chain Polyclonal Antibody, Unconjugated(bs-10124R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C. Western blot analysis of lysate from HepG2 cell line,using Fibrinogen beta chain Antibody(APR05478G). APR05478G was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;Incubation: Anti-Fibrinogen beta chain Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining. |
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說明書 |
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數(shù)量 |
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