CFL1 Antibody (N-term)

APR05089G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR05089G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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CFL1 Antibody (N-term)

品牌	Leading Biology	貨號	APR05089G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CFL1 Antibody (N-term).
分子量	18502 Da
細胞定位	Antigen Cellular Localization: Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide
宿主	Rabbit
種屬反應性	Human, Mouse
免疫原	4-32 aa
靶點	This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human CFL1.
亞型	Rabbit Ig
通用名	CFL
基因ID	1072
UniProt ID	P23528
功能	Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
總結	Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
圖像	Western blot analysis of CFL1 Antibody (N-term) (Cat. APR05089G) in MCF-7,Hela cell line and mouse brain tissue lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab.(2ug/ml) Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CFL1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of hela cells using CFL1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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