品牌 |
Leading Biology | 貨號(hào) |
APR05088G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACTR3 Antibody (C-term).
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分子量 |
47371 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton. Cell projection. Note=In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
380-407 aa
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靶點(diǎn) |
This ACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human ACTR3.
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亞型 |
Rabbit Ig
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通用名 |
ARP3
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基因ID |
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UniProt ID |
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功能 |
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis.
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總結(jié) |
ACTR3 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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圖像 |
Western blot analysis of ACTR3 Antibody (C-term) (Cat. APR05088G) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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