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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > Nestin Antibody (C-term)

Nestin Antibody (C-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
APR04529G 100 μl / ￥4950

Nestin Antibody (C-term)

品牌

 Leading Biology

貨號(hào)

 APR04529G

產(chǎn)品分類

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Nestin Antibody (C-term).

分子量
177439 Da

宿主
Rabbit

種屬反應(yīng)性
Human, Mouse

免疫原
1560-1590 aa

靶點(diǎn)
This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.

亞型
Rabbit Ig

基因ID

UniProt ID

功能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

總結(jié)
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
IHC-P, IF, E

稀釋方法
IHC-P~~1:50~100 IF~~1:10~50

圖像

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence analysis of anti-Nestin Antibody (C-term) (Cat.APR04529G) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.

說(shuō)明書(shū)

數(shù)量

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