品牌 |
Leading Biology | 貨號(hào) |
APR04022G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CD9 Antibody (N-term).
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分子量 |
25416 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
21-51 aa
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靶點(diǎn) |
This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human CD9.
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亞型 |
Rabbit Ig
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通用名 |
MIC3, TSPAN29
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基因ID |
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UniProt ID |
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功能 |
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
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總結(jié) |
CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
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圖像 |
Western blot analysis of CD9 Antibody (N-term) (Cat.APR04022G) in Jurkat cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab. Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (N-term) (Cat.APR04022G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies). Flow cytometric analysis of jurkat cells using CD9 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說(shuō)明書 |
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數(shù)量 |
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