品牌 |
Leading Biology |
貨號 |
APR03999G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TBX5 Antibody (N-term).
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分子量 |
57711 Da
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細胞定位 |
Antigen Cellular Localization:
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00201}
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
1-30 aa
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靶點 |
This TBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TBX5.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.
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總結(jié) |
This gene is a member of a phylogenetically conservedfamily of genes that share a common DNA-binding domain, the T-box.T-box genes encode transcription factors involved in the regulationof developmental processes. This gene is closely linked to relatedfamily member T-box 3 (ulnar mammary syndrome) on human chromosome12. The encoded protein may play a role in heart development andspecification of limb identity. Mutations in this gene have beenassociated with Holt-Oram syndrome, a developmental disorderaffecting the heart and upper limbs. Several transcript variantsencoding different isoforms have been described for this gene.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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圖像 |
All lanes : Anti-TBX5 Antibody (N-term) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: HepG2 whole cell lysates
Lane 3: HL-60 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 58 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
TBX5 Antibody (N-term) (Cat. APR03999G) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).
TBX5 Antibody (N-term) (APR03999G)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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