品牌 |
Leading Biology |
貨號 |
APR03998G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CD274 Antibody (C-term).
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分子量 |
33275 Da
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細胞定位 |
Antigen Cellular Localization:
Isoform 1: Cell membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
261-290 aa
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靶點 |
This CD274 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the C-terminal region of human CD274.
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亞型 |
Rabbit Ig
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通用名 |
B7H1, PDCD1L1, PDCD1LG1, PDL1
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基因ID |
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UniProt ID |
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功能 |
Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
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總結(jié) |
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:2000
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圖像 |
All lanes : Anti-CD274 Antibody (C-term) at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: HCT116 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Raji whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CD274 Antibody (C-term) at 1:2000 dilution + A549 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數(shù)量 |
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