UBE4A Antibody (Center)

APR03994G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR03994G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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UBE4A Antibody (Center)

品牌	Leading Biology	貨號	APR03994G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBE4A Antibody (Center).
分子量	122561 Da
細胞定位	Antigen Cellular Localization: Cytoplasm {ECO:0000250\|UniProtKB:E9Q735}.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	451-479 aa
靶點	This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A.
亞型	Rabbit Ig
基因ID	9354
UniProt ID	Q14139
功能	Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
總結(jié)	The modification of proteins with ubiquitin is animportant cellular mechanism for targeting abnormal or short-livedproteins for degradation. Ubiquitination involves at least threeclasses of enzymes: ubiquitin-activating enzymes, or E1s,ubiquitin-conjugating enzymes, or E2s, and ubiquitin-proteinligases, or E3s. This gene encodes an additional conjugationfactor, E4, which is involved in multiubiquitin chain assembly.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	All lanes : Anti-UBE4A Antibody (Center) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST. UBE4A Antibody (Center) (Cat. APR03994G) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the UBE4A antibody detected the UBE4A protein (arrow). UBE4A Antibody (Center) (APR03994G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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