Bcl-w Antibody (BH3 Domain Specific)

APR03770G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR03770G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Bcl-w Antibody (BH3 Domain Specific)

品牌	Leading Biology	貨號(hào)	APR03770G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Bcl-w Antibody (BH3 Domain Specific).
分子量	20746 Da
細(xì)胞定位	Antigen Cellular Localization: Mitochondrion membrane; Peripheral membrane protein. Note=Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	24-59 aa
靶點(diǎn)	This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-59 amino acids from human Bcl.
亞型	Rabbit Ig
通用名	BCLW, KIAA0271
基因ID	599
UniProt ID	Q92843
功能	Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
總結(jié)	Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
圖像	Western blot analysis of anti-Bcl-w BH3 domain Pab (Cat. APR03770G) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Western blot analysis of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody (Cat.APR03770G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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