Anti-IGHG1 Antibody (Center) at 1:2000 dilution + human plasma lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-IGHG1 Antibody (Center) at 1:2000 dilution
Lane 1: human fetal spleen lysate
Lane 2: human fetal thymus lysate
Lane 3: human spleen lysate
Lane 4: human plasma lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing HL-60 cells stained with APR03502G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR03502G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.