品牌 |
Leading Biology |
貨號(hào) |
APR03458G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HHLA2 Antibody (N-term).
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分子量 |
46850 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
58-87 aa
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靶點(diǎn) |
This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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圖像 |
All lanes : Anti-HHLA2 Antibody (N-term) at 1:2000 dilution
Lane 1: human pancreas lysate
Lane 2: MDA-MB-453 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
HHLA2 Antibody (N-term) (Cat. APR03458G) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHLA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
HHLA2 Antibody (N-term) (Cat. APR03458G) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說(shuō)明書(shū) |
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數(shù)量 |
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