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CNTF Antibody

貨號 貨期 規(guī)格 / 價格 詢價
APR11614G 50 μl / ￥6950

CNTF Antibody

品牌

 Leading Biology

貨號

 APR11614G

產品分類

 Polyclonal Antibodies

研究領域

產品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CNTF antibody.

分子量
22931 Da

宿主
Rabbit

種屬反應性
Human, Rat

靶點
Peptide (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441). C-terminus.

基因ID

UniProt ID

總結
CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1.It was initially purified from the chick eye on the basis of its ability topromote survival of E8 chick ciliary ganglion neurons in culture2.CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstratedthat CNTF is ubiquitously distributed in neurons and glia throughoutthe rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specificationsin hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, CNTF induces sprouting of cholinergicmotor neurons8 and delays neural degeneration in genetic modelsof motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.Abgent is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF (#AG1166) antibody can be used in western blotand immunocytochemical applications,and has been designed to recognize CNTF from human, rat and mouse samples. In addition, Anti-CNTF specifically recognizes CNTF but not related cytokines.

形式
Affinity purified antibody, Liquid

儲存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應用
WB

稀釋方法
WB~~1:200-1:2000 IHC~~1:200 ICC~~1:200

別名
Ciliary neurotrophic factor, CNTF, CNTF

圖像

Western blot analysis using Anti-CNTF on: 1. human CNTF ( C-240), 20ng. 2. hCardiotrophin-1 ( C-200), 20ng. 3. hLIF ( L-200), 20ng. 4. mIL-6 ( I-220), 20 ng. 5. human CNTF ( C-240), 20ng. Lanes 1-4. Anti-CNTF antibody ( APR11614G), (1:200). Lane 5. Anti-CNTF, preincubated with the control peptide antigen. The specificity of the antibody is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.

Expression of Ciliary Neurotrophic Factor (CNTF) in rat hippoccampus Immunohistochemical staining of rat hippoccampus using Anti-CNTF antibody ( APR11614G), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows show examples) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.

Expression of CNTF in rat C6 glioma cells Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF antibody ( APR11614G), (1:200), followed by goat anti-rabbit-DyLight-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

說明書

數(shù)量

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