DHCR7 Antibody (C-term)

APR07555G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR07555G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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DHCR7 Antibody (C-term)

品牌	Leading Biology	貨號	APR07555G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DHCR7 antibody (C-term).
分子量	H=54;M=54 KDa
細胞定位	Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
宿主	Rabbit
種屬反應性	Human, Mouse
免疫原	437-463 aa
靶點	This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.
通用名	D7SR
基因ID	1717
UniProt ID	Q9UBM7
功能	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
總結	This gene encodes an enzyme that removes the C(7-8) doublebond in the B ring of sterols and catalyzes the conversion of7-dehydrocholesterol to cholesterol. This gene is ubiquitouslyexpressed and its transmembrane protein localizes to theendoplasmic reticulum membrane and nuclear outer membrane.Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); asyndrome that is metabolically characterized by reduced serumcholesterol levels and elevated serum 7-dehydrocholesterol levelsand phenotypically characterized by mental retardation, facialdysmorphism, syndactyly of second and third toes, andholoprosencephaly in severe cases to minimal physical abnormalitiesand near-normal intelligence in mild cases. Alternative splicingresults in multiple transcript variants that encode the sameprotein.
形式	Liquid. In PBS containing 0.02% sodium azide.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB
稀釋方法	IF~~1:25 WB~~0.25
別名	7-dehydrocholesterol reductase, 7-DHC reductase, Putative sterol reductase SR-2, Sterol Delta(7)-reductase, DHCR7, D7SR
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP11452B at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Li-7 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: mouse testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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