品牌 |
Leading Biology |
貨號 |
APG01465G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACAT1 antibody (C-term).
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分子量 |
H=45.2 KDa
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細胞定位 |
Antigen Cellular Localization:
Mitochondrion.
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse
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免疫原 |
311-349 aa
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靶點 |
This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1.
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通用名 |
ACAT, MAT
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基因ID |
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UniProt ID |
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功能 |
Plays a major role in ketone body metabolism.
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總結(jié) |
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
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形式 |
Liquid. In PBS containing 0.02% sodium azide.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P
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稀釋方法 |
IHC-P~~1:10~50
WB~~1:1000
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別名 |
Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
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圖像 |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat. APG01465G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: mouse liver lysates
Lane 3: SK-BR-3 whole cell lysates
Lane 4: SW620 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數(shù)量 |
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