品牌 |
Leading Biology |
貨號(hào) |
APR17768G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GNL3 antibody (N-term).
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分子量 |
H=62, 61;M=61, 57;R=61 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
38-72 aa
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靶點(diǎn) |
This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 38-72 amino acids from the N-terminal region of human GNL3.
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通用名 |
E2IG3, NS
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基因ID |
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UniProt ID |
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功能 |
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
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總結(jié) |
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IF, WB
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稀釋方法 |
WB~~1:1000
IF~~1:25
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別名 |
Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
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圖像 |
All lanes : Anti-GNL3 Antibody (N-term) at 1:1000 dilution
Lane 1: F9 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: K562 whole cell lysates
Lane 4: Raji whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of Hela cells stained with GNL3 Antibody (N-term)( APR17768G). APR17768G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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說(shuō)明書(shū) |
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數(shù)量 |
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