YWHAG Antibody (N-term)

APR17892G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR17892G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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YWHAG Antibody (N-term)

品牌	Leading Biology	貨號	APR17892G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality YWHAG antibody (N-term).
分子量	H=28,M=28,Rat=28 KDa
細胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	63-92 aa
靶點	This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG.
基因ID	7532
UniProt ID	P61981
功能	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
總結(jié)	YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IHC-P
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
別名	YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; 14-3-3 protein gamma, N-terminally processed
圖像	Western blot analysis of lysates from Hela,A431 cell line,mouse brain tissue lysate(from left to right), using YWHAG Antibody (N-term)(Cat. APR17892G). APR17892G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. YWHAG Antibody (N-term) (Cat. APR17892G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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