品牌 |
Leading Biology |
貨號 |
AMM05102G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Goat Anti-Pyruvate Carboxylase antibody.
|
分子量 |
129634 Da
|
細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
|
宿主 |
Goat
|
種屬反應(yīng)性 |
Mouse
|
亞型 |
IgG
|
基因ID |
|
UniProt ID |
|
功能 |
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
|
總結(jié) |
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
|
形式 |
0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, E
|
別名 |
Pyruvate carboxylase, mitochondrial, 6.4.1.1, Pyruvic carboxylase, PCB, PC
|
圖像 |
AMM05102G (0.03 μg/ml) staining of Mouse Liver lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Human Pyruvate Carboxylase (RC221895) and probed with AMM05102G (mock transfection in first lane), tested by Origene.
|
說明書 |
|
數(shù)量 |
|