品牌 |
Leading Biology |
貨號 |
APR17541G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NCOA7 antibody (N-term).
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分子量 |
106162 Da
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細胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse
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免疫原 |
76-103 aa
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靶點 |
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
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亞型 |
Rabbit Ig
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通用名 |
ERAP140, ESNA1
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基因ID |
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UniProt ID |
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功能 |
Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
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總結 |
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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別名 |
Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1, NCOA7, ERAP140, ESNA1
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圖像 |
All lanes : Anti-NCOA7 Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: human brain lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 106 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
NCOA7 Antibody (N-term) (Cat. APR17541G) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).
Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數量 |
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