品牌 |
Leading Biology |
貨號 |
APR11837G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ENTPD2 antibody (N-term).
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分子量 |
53665 Da
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細胞定位 |
Antigen Cellular Localization:
Isoform Long: Cell membrane; Multi-pass membrane protein Isoform gamma: Endoplasmic reticulum membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
86-113 aa
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靶點 |
This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
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亞型 |
Rabbit Ig
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通用名 |
CD39L1
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基因ID |
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UniProt ID |
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功能 |
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP.
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總結(jié) |
ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Ectonucleoside triphosphate diphosphohydrolase 2, NTPDase 2, 361-, CD39 antigen-like 1, Ecto-ATP diphosphohydrolase 2, Ecto-ATPDase 2, Ecto-ATPase 2, ENTPD2, CD39L1
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圖像 |
All lanes : Anti-ENTPD2 Antibody (N-term) at 1:2000 dilution
Lane 1: H.heart lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: H.brain whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-ENTPD2 Antibody at 1:1000 dilution
Lane 1: human heart lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: human brain lysate
Lane 5: human skeletal muscle lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ENTPD2 Antibody (N-term) (Cat. APR11837G) in K562 cell line lysates (35ug/lane). ENTPD2 (arrow) was detected using the purified Pab.
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說明書 |
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數(shù)量 |
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