品牌 |
Leading Biology | 貨號 |
APR14966G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ANXA7 antibody (Center).
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分子量 |
52739 Da
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
329-356 aa
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靶點 |
This ANXA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the Central region of human ANXA7.
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亞型 |
Rabbit Ig
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通用名 |
ANX7, SNX
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基因ID |
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UniProt ID |
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功能 |
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
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總結(jié) |
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. ANXA7 is a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Annexin A7, Annexin VII, Annexin-7, Synexin, ANXA7, ANX7, SNX
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圖像 |
ANXA7 Antibody (Center) (Cat. APR14966G) western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the ANXA7 antibody detected ANXA7 protein (arrow). (LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of ANXA7 Antibody (Center) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI. ANXA7 Antibody (Center) (Cat. APR14966G) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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