IRS2 Antibody (C-term)

APR08046G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR08046G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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IRS2 Antibody (C-term)

品牌	Leading Biology	貨號	APR08046G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IRS2 antibody (C-term).
分子量	137334 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm, cytosol.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1309-1338 aa
靶點(diǎn)	This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
亞型	Rabbit Ig
基因ID	8660
UniProt ID	Q9Y4H2
功能	May mediate the control of various cellular processes by insulin.
總結(jié)	Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
別名	Insulin receptor substrate 2, IRS-2, IRS2
圖像	Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)( APR08046G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).IRS2 immunoreactivity is localized to Cytoplasm significantly. Western blot analysis of anti-IRS2 Antibody (C-term) (Cat. APR08046G) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.
說明書	Product Datasheet Protocol
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