品牌 |
Leading Biology |
貨號 |
AMM06800G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NPTX1-Y344 antibody.
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分子量 |
47122 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasmic vesicle, secretory vesicle
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat
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免疫原 |
322-350 aa
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靶點(diǎn) |
This NPTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from human NPTX1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
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總結(jié) |
NPTX1 is a member of the neuronal pentraxin family. Human neuronal pentraxin 1 is similar to rat NP1 which is a binding protein for the snake venom toxin taipoxin.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:1000
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別名 |
Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1
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圖像 |
AMM06800G staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AMM06800G staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-NPTX1-Y344 Antibody at 1:2000 dilution
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lane 3: rat brain lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數(shù)量 |
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