PGK1 Antibody (Center)

AMM04370G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM04370G		100 μl / ￥4950

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PGK1 Antibody (Center)

品牌	Leading Biology	貨號(hào)	AMM04370G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PGK1 antibody (Center).
分子量	44615 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	117-145 aa
靶點(diǎn)	This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
亞型	Rabbit Ig
通用名	PGKA
基因ID	5230
UniProt ID	P00558
功能	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
總結(jié)	Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, FC, E
稀釋方法	IHC-P~~1:10~50 WB~~1:1000 IF~~1:10~50 FC~~1:10~50
別名	Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
圖像	Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center)( AMM04370G). AMM04370G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132)( AMM04370G). AMM04370G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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