Villin-1 Antibody (N-term)

AMM08466G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM08466G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Villin-1 Antibody (N-term)

品牌	Leading Biology	貨號	AMM08466G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Villin-1 antibody (N-term).
分子量	92695 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip. Cell projection, filopodium. Note=Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	180-207 aa
靶點(diǎn)	This Villin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-207 amino acids from the N-terminal region of human Villin-1.
亞型	Rabbit Ig
通用名	VIL
基因ID	7429
UniProt ID	P09327
功能	Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
總結(jié)	Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
別名	Villin-1, VIL1, VIL
圖像	All lanes : Anti-Villin-1 Antibody (N-term) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Villin-1 Antibody (N-term) (Cat. AMM08466G) western blot analysis in HT29 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the Villin-1 antibody detected the Villin-1 protein (arrow). Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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