品牌 |
Leading Biology | 貨號(hào) |
APG01617G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AGXT antibody (Center).
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分子量 |
43010 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Peroxisome. Mitochondrion matrix. Note=Except in some HP1 patients where AGT is found in the mitochondrial matrix
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
96-125 aa
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靶點(diǎn) |
This AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.
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亞型 |
Rabbit Ig
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通用名 |
AGT1, SPAT
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基因ID |
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UniProt ID |
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總結(jié) |
AGXT is expressed only in the liver and protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
IHC-P~~1:50~100
WB~~1:1000
FC~~1:10~50
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別名 |
Serine--pyruvate aminotransferase, SPT, Alanine--glyoxylate aminotransferase, AGT, AGXT, AGT1, SPAT
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圖像 |
APG01617G staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-AGXT Antibody (Center)at 1:8000 dilution + human liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說明書 |
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數(shù)量 |
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