SLC10A1 Antibody (C-term)

APR09991G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APR09991G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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SLC10A1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR09991G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SLC10A1 antibody (C-term).
分子量	38119 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Multi-pass membrane protein.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	316-343 aa
靶點(diǎn)	This SLC10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-343 amino acids from the C-terminal region of human SLC10A1.
亞型	Rabbit Ig
通用名	NTCP
基因ID	6554
UniProt ID	Q14973
功能	The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
總結(jié)	Sodium/bile acid cotransporters are integral membraneglycoproteins that participate in the enterohepatic circulation ofbile acids. Two homologous transporters are involved in thereabsorption of bile acids, one absorbing from the intestinallumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateralmembranes of hepatocytes (SLC10A1).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	FC~~1:25 WB~~1:2000
別名	Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, SLC10A1, NTCP
圖像	Overlay histogram showing K562 cells stained with APR09991G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR09991G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說(shuō)明書(shū)	Product Datasheet Protocol
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