PRDX2 Antibody (C-term)

AMR09501G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
AMR09501G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PRDX2 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	AMR09501G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PRDX2 antibody (C-term).
分子量	21892 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	169-198 aa
靶點(diǎn)	This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.
亞型	Rabbit Ig
通用名	NKEFB, TDPX1
基因ID	7001
UniProt ID	P32119
功能	Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
總結(jié)	PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
別名	Peroxiredoxin-2, Natural killer cell-enhancing factor B, NKEF-B, PRP, Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2, NKEFB, TDPX1
圖像	Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody (C-term) ( AMR09501G). AMR09501G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Fluorescent confocal image of Hela cell stained with PRDX2 Antibody (C-term)( AMR09501G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRDX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly. Western blot analysis of PRDX2 Antibody (C-term) (Cat. AMR09501G) in MDA-MB231,293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab.(2ug/ml)
說(shuō)明書(shū)	Product Datasheet Protocol
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