品牌 |
Leading Biology | 貨號 |
APR07706G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ENOB antibody (Center).
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分子量 |
46987 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
237-264 aa
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靶點(diǎn) |
This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Appears to have a function in striated muscle development and regeneration.
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總結(jié) |
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, ENO3
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圖像 |
ENOB Antibody (Center) (Cat. APR07706G) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow). Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. ENOB Antibody (Center) (Cat. APR07706G) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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