ENPP2 Antibody (Center)

APR07713G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07713G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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ENPP2 Antibody (Center)

品牌	Leading Biology	貨號(hào)	APR07713G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ENPP2 antibody (Center).
分子量	98994 Da
細(xì)胞定位	Antigen Cellular Localization: Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	377-406 aa
靶點(diǎn)	This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
亞型	Rabbit Ig
通用名	ATX, PDNP2
基因ID	5168
UniProt ID	Q13822
功能	Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
總結(jié)	ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50
別名	Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2
圖像	Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center)( APR07713G). APR07713G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center) ( APR07713G). 293T cell lysates (２ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene. Western blot analysis of ENPP2 (arrow) using rabbit polyclonal ENPP2 Antibody (Center) (Cat. APR07713G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ENPP2 gene (Lane 2) (Origene Technologies).
說明書	Product Datasheet Protocol
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