品牌 |
Leading Biology |
貨號 |
AMM06343G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MATH1/HATH1/ATOH1 antibody (N-term).
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分子量 |
38160 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
78-109 aa
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靶點(diǎn) |
This MATH1/HATH1/ATOH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-109 amino acids from the N-terminal region of human MATH1/HATH1/ATOH1.
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亞型 |
Rabbit Ig
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通用名 |
ATH1, BHLHA14
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基因ID |
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UniProt ID |
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功能 |
Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription (By similarity).
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總結(jié) |
ATOH1 belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
Protein atonal homolog 1, Class A basic helix-loop-helix protein 14, bHLHa14, Helix-loop-helix protein hATH-1, hATH1, ATOH1, ATH1, BHLHA14
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圖像 |
All lanes : Anti-ATOH1 (Human N-term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: human fetal brain lysate
Lane 6: mouse brain lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 38 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ATOH1 (arrow) using rabbit polyclonal ATOH1 Antibody (Human N-term) (Cat. AMM06343G).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATOH1 gene (Lane 2) (Origene Technologies).
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說明書 |
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數(shù)量 |
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