品牌 |
Leading Biology | 貨號 |
APR14382G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PSMB7 antibody (Center).
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分子量 |
29965 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809, ECO:0000269|PubMed:18549262}. Nucleus
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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靶點 |
This PSMB7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 180-211 amino acids from the Central region of human PSMB7.
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亞型 |
Rabbit Ig
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通用名 |
Z
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基因ID |
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UniProt ID |
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功能 |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
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總結(jié) |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:8000
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別名 |
Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z
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圖像 |
APR14382G staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-PSMB7 Antibody (Center) at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: Hela whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilution Lane 1: Jurkat whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: MCF-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說明書 |
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數(shù)量 |
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