RNF7 Antibody (N-term)

AMR09751G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMR09751G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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RNF7 Antibody (N-term)

品牌	Leading Biology	貨號	AMR09751G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RNF7 antibody (N-term).
分子量	12683 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
靶點	This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7.
亞型	Rabbit Ig
通用名	RBX2, ROC2, SAG
基因ID	9616
UniProt ID	Q9UBF6
功能	Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
總結(jié)	Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IHC-P, WB, E
稀釋方法	IHC-P~~1:25 WB~~1:8000
別名	RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG
圖像	AMR09751G staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-RNF7 Antibody (N-term) at 1:8000 dilution Lane 1: human heart lysates Lane 2: human liver lysates Lane 3: human skeletal muscle lysates Lane 4: human testis lysates Lane 5: mouse heart lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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